Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPLYVTSGEPAGIGPDICLSLAKRV-DERPVVVLADRNLLHQRAQKLGLDIKFLEYS-GQAESS-LQGELYIEHVPLETIVVDGQLNAANAAYVLEQLRRSADYAMSGKSVGVATAPVQKSVINDAGILFSGHTEYYQEFAGVERVVMMLATKTLRVALATTHLPLRDVADAITKERLHQVIDILLRDLKTKFKINDPRVLVCGLNPHAGEDGYLGREEIDTINPVLESYRSQGVKLSLSLPADTLFTPEHLKNADAVLAMYHDQGLPVLKSQGFGEAINITLGLPFIRTSVDHGTALSLAGTGLAKSSSLNVAADLALSLAAS 1PTM Chain:A ((4-327)) TQRVVITPGEPAGIGPDLVVQ-LAQREWPVELVVCADATLLTNRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLARACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRVALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHMGTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQGFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVN

General information: TITO was launched using: RESULT: Template: 1PTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1850 -29635 -16.02 -92.32 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -16.02 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.608

(partial model without unconserved sides chains): PDB file : Tito_1PTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PTM-query.scw PDB file : Tito_Scwrl_1PTM.pdb: