Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKATQSKAWTTVQIARHPERPQ-FLDYVGEIFTE--FDALHGDRLFGDDGAMVGGLARFDGQPVMVIGQHRGRSTREKLKHNFGMCNPEGYRKSQRLLDMAERFNLPVFTFIDTMGAYPGVGAEERGQAEAIATSLAQLSSLKVPVIATVLGEGGSGGALGIG----VADRVIMLSHSIYSVISPEGCASILWKTAEKAAQASEA----L--GLTADKLQSLGIVEYVVDEGEGAHLDPERVMQNLKVVLKQALDELLPMDANERCEARYQRLMKFGSENLGMAS 5ING Chain:A ((328-555)) ---------------PDSEQQVYDMADVITAVLDDGDYLEIHPD--FA--RNIICALGRVEGHSVAVVANQP--------RHLAGVLDIDASEKAARFIRFCDSFNIPVLTFMDVPGYLPGVGQEHQGIIRRGIKLFYAYAESTVPKITVITRKAYGGGYAVMGSRQIGADRVMAWPTAEIAVMGANSAVPIL-------------VDDYRRRFGNPYEAAAHGYVDMVIS-P-----------SRTRYEVARALASLRN--------------------------

General information: TITO was launched using: RESULT: Template: 5ING.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1032 -18860 -18.28 -96.72 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -18.28 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_5ING.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ING-query.scw PDB file : Tito_Scwrl_5ING.pdb: