Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILFTLRKKGGKRYMNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVIKETDHVYHLGDLA---MHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPRLTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPI---ENDVNVGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS--------------------------------- 2HY1 Chain:A ((12-267)) ---------PRPDYVLLHISDTHL----------------IDADDRLGELLEQLNQSGLRPDAIVFTGDLADKGEPAAYRKLRGLVEPFAAQLGAELVWVMGNHDDRAELRKFLLDEAPSMAPLDRVCMIDGLRIIVLDTSVPGHHHGEIRASQLGWLAEELATPAPDGTILALHHPPIPSVLDMAVTVELRDQAALGRVLRGTDVRAILAGHLHYSTNATFVGIPVSVASATCGCNLVHVYPDTVVHSVIP

General information: TITO was launched using: RESULT: Template: 2HY1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 938 -14503 -15.46 -77.14 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -15.46 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_2HY1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HY1-query.scw PDB file : Tito_Scwrl_2HY1.pdb: