Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMNIEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSKLVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGKGIRYVGEYVRRKEGKTGK 2J28 Chain:G ((1-176)) -SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAKKK-

General information: TITO was launched using: RESULT: Template: 2J28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 763 -54005 -70.78 -306.85 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain G : 0.84 3D Compatibility (PKB) : -70.78 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_2J28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J28-query.scw PDB file : Tito_Scwrl_2J28.pdb: