Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASNAAANGSFGSSANAVQLVSTQRGRKVYCVRGQSFDIDDNYTVTSVIGHGAYGVVCAALDDRTFQEVAIKRVSRVFEDLIDGRRIWREILLLRILKECGCRNVLRLIRVLPPRDPIMEFRDLYLVTDLYDIDLFSIIRQNKCESIDLLRRISVRVLRCLADMHSMGIVHRDIKPSNILLRDEKNAEEAIVCDFGLARAGLHRLSEPLDLTDYVVTRWYRPPELLLM-CPYSYPIDIWAVGCVMAEYAMQRPLFAGRDYIHQLQFVLSSIPITGVDFIERSSSSSGLANMNEIAKKYKGTRPLPQLLSKLPKDGLELVTEMLAFEPNKRITAQEALKHPFFSSVGGPDCKSYPAPPELDLGFDMHAEVSECQLRRAIWDELQYYRK 2FST Chain:X ((25-351)) ------------------------------------WEVPERYQNLSPVG----GSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRL---LKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLN------QKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCE---LKILD---------------------ATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQM--PKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADP--YDQSLESR-DLLIDEWKSLTYDE------

General information: TITO was launched using: RESULT: Template: 2FST.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 1396 -135086 -96.77 -447.30 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain X : 0.76 3D Compatibility (PKB) : -96.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2FST.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FST-query.scw PDB file : Tito_Scwrl_2FST.pdb: