Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------RPSKGRLKRVVI---------CEVFIRMSGGLDLPIGKDFVPAGETTKIHAYTCET------------DTMC-----GAEC----DTPSNYRYSSWVLERELRDWEKARLRARLALASSPESRERANMLVKLLC-------- 3WEB Chain:A ((1-132)) FVFEDCGSEVGKFSDIIISSCDPSEEKCSII----RESEIHVSMKFTPSVDVKNVEAKAFGVLLDVPVPFPLKKPEICKDPDSGVKCPLKKDVEIEYKVTFFV------EKATPALSLEIMWEFRNEKDE------KITCVKFPAKIK

General information: TITO was launched using: RESULT: Template: 3WEB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 228 8320 36.49 94.54 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 36.49 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.077

(partial model without unconserved sides chains): PDB file : Tito_3WEB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WEB-query.scw PDB file : Tito_Scwrl_3WEB.pdb: