Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------QVFGCFVQMSVRTQDSKDETNISSGGYLLTTNPGQLVFTDG-----------------NNNITVKVDKD-------CQSAGISYHR-----SEVHFTA-LQKLSENGGPFEFDQPLIELQ 4QQD Chain:A ((133-281)) GLYKVNEYVDARDTNMGAWFEAQVVRVTRKEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIE

General information: TITO was launched using: RESULT: Template: 4QQD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 299 1390 4.65 15.44 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 4.65 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_4QQD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QQD-query.scw PDB file : Tito_Scwrl_4QQD.pdb: