Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------KCNISILKDGKYF--TQKQEIAG---WTVK---FHDLDGNYVGKCKNAH--TYACTYVTCE--DLAPGY----SPGKS------KDVVAS-GN--------VGSLGHDPSSS-------SPSSP-------------------------------------- 2PTH Chain:? ((1-193)) TIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAKFFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIG-IGHPGDKNKVVGFVLGKPPVSEQKLIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ

General information: TITO was launched using: RESULT: Template: 2PTH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 334 24637 73.76 289.85 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 73.76 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_2PTH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PTH-query.scw PDB file : Tito_Scwrl_2PTH.pdb: