Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLLHPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALIIAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVLAALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLTRTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSFFTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIGIF 2L9U Chain:A ((637-676)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGRTHLTMALTVIAGLVVIFMMLGGTFLYWRGRRHHHHHH---------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2L9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 12 -1938 -161.46 -48.44 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.38 3D Compatibility (PKB) : -161.46 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.38 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_2L9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L9U-query.scw PDB file : Tito_Scwrl_2L9U.pdb: