Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVT-----QNVESLLVSIVISAYNEEKYLPGLIE---DLKNQTYP----------KEDIEILFINA------MSTDGTTAIIQQF-----IKEDTEFNSIRLYNNPKKNQASGFNLG-VKHSVGDLILKIDAHS-----KVTESFVMNNVAIIQQGEFVCGGPRPTIVEGKGKWAE--TLHLVEENMFGSSIANYRN----------SSEDRYVSSIFHGMYKREVFQKV--------------GLVNEQLGRTEDNDIHYRIREHGYKIRYS-PSILSYQYIRPTF----------------------------------------------------KKMLHQKYSN-GLWIGLTSHVQ----FKCLSLFHYVP------CLFVLSLVFSLALLPITFVFITLLL-GAYFLLLSLLTLLTLLKHKNGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTIIYLDKISQINKNML 5I62 Chain:A ((1-534)) GQVAPNVWSKYFNIPNPGLRAYFSNVVSGQPEVYRTPFYKGMSLESICDEWYKKLVSIDTQWPTLMEFEDDLRKKVGPMSVMLPLKERMSDIDSYYDSISKDQVPFDTKAISAAKSEWKGVSRLRLRSEVNTVAVM---KKSTNSGSPYFSKRKAVVSKTIPCDVYMDGRYCVMRQNGREWSGAAVLGWRGQEGGPKPTDVKQRVVWMFPFAVNIRELQVYQPLILTFQRLGLVPAWVSMEAVDRRITKMFDTKGPRDVVVCTDFSKFDQHFNPTCQSVAKELLADLLTGQEAVDWLERVFPIKYAIPLAYNWGEIRYGIHGMGSGSGGTNADETLVHRVLQHEAAISHHTTLNPNSQCLGDDGVLTYPGISAEDVMQSYSRHGLDMNLEKQYVSKQDCTYLRRWHHTDYRVDGMCVGVYSTMRALGRLAMQERYYDPDVWGEKMVTLRYLSIIENVKYHPLKEEFLDFCIKGDKTRLGLG-IPGFLDNIAGEAQKGIENWWVVQALKSRR---

General information: TITO was launched using: RESULT: Template: 5I62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1792 -35872 -20.02 -94.90 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -20.02 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_5I62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I62-query.scw PDB file : Tito_Scwrl_5I62.pdb: