Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEIKRIQQQPDLAQDIYAVMAAVYPVSPWTLEQIQADLSQDQTWYALAYDGAEVIGFLTVQETLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKE------IFLEVRQSNQRAQAFYKKEKMAVIAERKAYYHDPV----EDAIIMKREIDEG 2CNM Chain:C ((53-142)) --------------------------------------------------------AFAITQVVLDEATLFNIAVDPDFQRRGLGRMLLEHLIDELETRGVVTLWLEVRASNAAAIALYESLGFNEATIRRNYY--PTAQGHEDAIIM-------

General information: TITO was launched using: RESULT: Template: 2CNM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 229 -19549 -85.37 -244.36 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -85.37 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_2CNM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CNM-query.scw PDB file : Tito_Scwrl_2CNM.pdb: