Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------MDIRQVTETIAMIEEQNFDIRTITMGISLLDC---IDPNIN---RAAEK-------IYQKITTKAANLVAVGDEIAAELGIPIVNKRVS----------VTPISLIGAA------TDATDYVVLAKALDKAAK--EIGVDFIGGFSALVQKGYQKGDEILINSIPRALAE--TDKVCSSVNIGSTKSG----INMTAVADM----------------------------------------------------------GRIIKETANLSDMGVAKLVVFA--NAVEDNPFMAGAFHGV---------------GEADVIINVGVSGPGVVKRALEKVRGQSFDVVAETVKKTA-----FKITR-----------IGQLVGQMASERLGVEFGIVDLSLAPTPAVGDSVARVLEEMGLETV--GTHGTTAALALLNDQVKKG-GVMACNQV--GGLSGAFIPVSEDEGMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIG--------VINMKTTAVRIIPKGREGDMIEFGGLL------GTAPVMKVNG-------------------ASSVDFISRGGQIPAPIHSFKN 4M1K Chain:A ((6-688)) EYDLKRLRNIGIAAHIDAGKTTTTERILYYTGRIAAVTTCFWKDHRINIIDTPGHVDFTIEVERSMRVLDGAIVVFDSSQGVEPQSETVWRQAEKYKVPRIAFANKMDKTGADLWLVIRTMQERLGARPVVMQLPIGREDTFSGIIDVLRMKAYTYGNDLGTDIREIPIPEEYLDQAREYHEKLVEVAADFDENIMLKYLEGEEPTEEELVAAIRKGTIDLKITPVFLGSALKNKGVQLLLDAVVDYLPSPLDIPPIKGTTPEGEVVEIHPDPNGPLAALAFKIMADPYVGRLTFIRVYSGTLTSGSYVYNTTKGRKERVARLLRMHANHREEVEELKAGDLGAVVGLKETITGDTLVGEDAPRVILESIEVPEPVIDVAIEPKTKADQEKLSQALARLAEEDPTFRVSTHPETGQTIISGMGELHLEIIVDRLKREFKVDANVGKPQVAYRETITKPVDVEGKFIRLTGGRGQYGHVKIKVEPLPRGSGFEFVNAIVGGVIPKEYIPAVQ-KGIEEAMQSGPLIGFPVVDIKVTLYDGSYHEVDSSEMAFKIAGSMAIKEAVQKGDPVILEPIMRVEVTTPEEYMGDVIGDLNARRGQILGMEPRGNAQVIRAFVPLAEMFGYATDLRSKTQGRGSFVMFFDHYQEVPKQVQEKLI

General information: TITO was launched using: RESULT: Template: 4M1K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2457 78666 32.02 177.18 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 32.02 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_4M1K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M1K-query.scw PDB file : Tito_Scwrl_4M1K.pdb: