Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIG--FGPGTLMAISDHINM---TGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKL--NIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEEL---NHEEVVEVTERVKGDFKGLLKAILAEL 3K8O Chain:U ((13-282)) ---------TAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASI

General information: TITO was launched using: RESULT: Template: 3K8O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain U - contact count / total energy / energy per contact / energy per residue : 1430 -96469 -67.46 -371.03 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain U : 0.83 3D Compatibility (PKB) : -67.46 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_3K8O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K8O-query.scw PDB file : Tito_Scwrl_3K8O.pdb: