Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEANNTTI-------------IGYCLRHQEQGK----HIITTAIEHHAVLETIDYLVQHFGFEATIIQPENQ---EITAQQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILKQ----------HPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSM----DFDSYLHGGDQEQKKRAGTENLPAIVGMVAA---LKENLEKQEEHFQHVQN-LETAFLAELEGIQYYLNR---GKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG 3GZD Chain:B ((27-433)) -VYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRKAKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTS------------GAKPHFITSSVEHDSIRLPLEHLVEEQVAAVTFV-PVSKVSGQTEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEISQRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPR-IGALYIRGLGEFTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFPGTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVG--------QPSPVLLS-YGVPFDVARNALRLSVGRSTTRAEVDLVVQDLKQAV--

General information: TITO was launched using: RESULT: Template: 3GZD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1871 8101 4.33 23.48 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 4.33 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_3GZD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GZD-query.scw PDB file : Tito_Scwrl_3GZD.pdb: