Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVEEEKAFLARHLKATEAGEFVTIDALFQAYKKELGRSYTRDAFYQLLKRHGWRNITPRPEHPRKADAQTIVASKNKISIQEDKKAL 2LBF Chain:A ((1-69)) MASVSELACIYSALILHDDEVTVTEDKINALIKAA-GVNVEPFWPGLFAKALANVNIGSLICNVGAGGPA------------------

General information: TITO was launched using: RESULT: Template: 2LBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 229 -29494 -128.79 -427.44 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -128.79 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_2LBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LBF-query.scw PDB file : Tito_Scwrl_2LBF.pdb: