Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVLLFLFSGGKMAKELQSSCYIVISFLVREMGIDIVEAISLMAELEKSGLVRLESSGDLILKELGGAL 2JSC Chain:B ((34-74)) ----------------------VCYPGQLAAHLGLTRSNVSNHLSCLRGCGLVVATYEGRQVR-------

General information: TITO was launched using: RESULT: Template: 2JSC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 87 -16934 -194.64 -413.01 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -194.64 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_2JSC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JSC-query.scw PDB file : Tito_Scwrl_2JSC.pdb: