Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNA-REIEVGLLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI 2YZN Chain:C ((3-317)) ----VLLIAGGVSPEHEVSLLSAEGVLRHIP---FPTDLAVIAQDGRWLLGEKALTA------LEAKAAPEGEHPFPPPLSWERYDVVFPLLHGRFGEDGTVQGFLELLGKPYVGAGVAASALCMDKDLSKRVLAQAGVPVVPWVAVRKGEPPVVPFDPPFF-------VKPANTGSSVGISRVERFQDLEAALALAFRYDEKAVVEKALSPVRELEVGVLGNVFGEASPVGEVRYEA----------PGRAELLIPAPLDPGTQETVQELALKAYKVLGVRGMARVDFFLAE-GELYLNELNTIPGFTPTSMYPRLFEAGGVAYPELLRRLVELA------------

General information: TITO was launched using: RESULT: Template: 2YZN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1730 -87151 -50.38 -286.68 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -50.38 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2YZN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YZN-query.scw PDB file : Tito_Scwrl_2YZN.pdb: