Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCPECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYHCSKMMVAETSIVKKNHQIPRI--INQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHD--FSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEGRTQAVIRDH---------FLKYDRAVRCRVKIITMDMFSPYYGLAKQLRFHIVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKF------FGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKPEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA 4P4M Chain:A ((92-378)) --------------------------------------------------------------------------------------------KPKLKAIQELTQVHGFGPRAAAALFDREGIFTVDELLQKADS--IPSLTDQQRVGIKYFYDINEKIPMQESVLHENYLREKCMEVLGKDFSILICGSYR---RRHPFSGDVDAILSRTLDAPPLSEPVAATGVLGHFVEFLESLKY-LEATMAQGPLKYMGMGRLPPRINTKVYKARRVDIRLIE---------TKSVPTAMLTFTGSKNFNVIMR-QAAISKGYLLNEYGLFKLGTPEEARGKNAGEELGVPKDELED--------KRVEVRSEQDVFDVLGMPY--AKPENRDP----------------------------------------

General information: TITO was launched using: RESULT: Template: 4P4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 19839 18.47 79.04 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 18.47 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_4P4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P4M-query.scw PDB file : Tito_Scwrl_4P4M.pdb: