Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPEGEIKGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDEG--HGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVGLRHY-------------------------------------KTPEIQGDILLIHGEQDDITLLSDAIEWAK-PQKHPITILSGANHFFTGYLKQLRQIITRFIIMK--- 2WTM Chain:A ((2-251)) ---------GAMYIDCDGIKLNAYLDMPKNNPEKCPLCIIIHGFTGHSEERHIVAV-QETLNEIGVATLRADMYGHGKSDGKFEDHTLFKWLTNILAVVDYAKKLDFVTDIYMAGHSQGGLSVMLAAAMERDIIKALIPLSPAAMIPEIARTGELLGLKFDPENIPDELDAWDGRKLKGNYVRVAQTIRVEDFVDKYTKPVLIVHGDQDEAVPYEASVAFSKQYKNCKLVTIPGDTHCYDHHLELVTEAVKEFMLEQIAK

General information: TITO was launched using: RESULT: Template: 2WTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1148 -49018 -42.70 -236.80 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -42.70 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2WTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WTM-query.scw PDB file : Tito_Scwrl_2WTM.pdb: