Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIYLNGELTDTPSQ----NLLQLIQELALEGK------RFAVEHNQQIVPKSKLEQISIAQHDRIEIIHAVGGG 3RPF Chain:C ((3-74)) VEVRFFGPIKEENFFIKANDLKELRAILQEKEGLKEWLGVCAIALNDHLIDN---LNTPLKDGDVISLLPPVCGG

General information: TITO was launched using: RESULT: Template: 3RPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 167 822 4.92 13.25 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : 4.92 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.597

(partial model without unconserved sides chains): PDB file : Tito_3RPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RPF-query.scw PDB file : Tito_Scwrl_3RPF.pdb: