Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLYIWMPETNGVWHWSNGENWLQAASLDQLIQDLQIHQGKEAAVFFPS---RHAQMLQQTMAKFHYKQLGADGVKYLLEEFVTLPIDHMK---VVHHFHA-----DQLTVLAV-------AQGMVETWQHSLALLPTKLVALLPDFLVLPEPQAQQVILCNIGHQLLVRENKWLGNSIDDLGL-FLEFQSAETHYQYSGLTAEQLESLEAASSAEQRSEFVYQFQPLDKTKQHPFNVLPKSKGQERTFSNYWKACAAVVLAIIVVQFSYDLLRWVKLKKVADQTAEQAIEQYKYWFGSSSGRVTEQNVRSNFKSRLEVSQQGDTQALSLLSRVGPILMQRQILAQQLNYDASILTMALKTKSADDLQALTQQLNQQGFKAELGNVQADGSGAIGVVKIQ 3LZC Chain:A ((1-342)) MLHEI--------------------PKSEILKELKRIGAKRVLIQSPEGLRREAEELAGFLEENNIEVFLHGEINYGACDPADREAKLVGCDALIHLGHSYMKLPLEVPTIFVPAFARVSVVEALKENIGEIKKLGRKIIVTTTAQHIHQLKEAKEFLESEGFEVSIGRGDSRISWPGQVLGCNYSVAKVRGEGILFIGSGIFHPLGLAVATRK-----KVLAIDPYTKAFSW----IDPERFIRKRWAQIAKAMDAKKFGVIVSIKKGQ-----LRLAEAKRIVKLLKKHGREARLIVMNDVNYHKLEGF-----------PFEAYVVVACPRVPLDDYGAWRKPVL---TPKEVEILLGLREEYEFDEILGGPRESDEPFGISIHSTR---------

General information: TITO was launched using: RESULT: Template: 3LZC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1702 6727 3.95 20.83 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 3.95 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_3LZC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LZC-query.scw PDB file : Tito_Scwrl_3LZC.pdb: