Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHLAQRLAAVVLTLGLSAGLVGCGFHLKGTNPTATPLVYKKLSL-ELPAKTDDLETQLKVYLTANGVQLSND----NDAYVLRVLEYTPRRQLL----NGKLTEVLLRLTVTFQIEDRQGNKITEPRTLTAARSYQYDLATVNTENQQESYLQRIVIDDLAQQITRQISANRLPKAQP 4Q35 Chain:B ((2-149)) -----------------------GWHLRDTT-QVP-STMKVMILDSGDPN-GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIMTVNATVLIP-GRDIY-PISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLPSIRAADI--

General information: TITO was launched using: RESULT: Template: 4Q35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 532 19624 36.89 141.18 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 36.89 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_4Q35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q35-query.scw PDB file : Tito_Scwrl_4Q35.pdb: