Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIEDVVKLGPVIPVLAFDSAEQGEH---VSRALHAGGVKVLEITLRTAAGLAAIERASQLADDIVVGVGTITKPEHCAQAKKAGAKFGVSPGLTKDLHLAAQDAGLPLLPGVMTPSDLIQAIELGYDIVKFFPAQQAGGVEMLKAFYGPFPNLRFCPTGGITAETAPDFLKQPNVVCVGGSWLTPKPVVAAQDWAEITRLAQIASQL 1FWR Chain:B ((27-209)) -------------------------EHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFQFFPAEANGGVKALQAIAGPFSQVRFCPKGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVE-

General information: TITO was launched using: RESULT: Template: 1FWR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 863 -5641 -6.54 -31.34 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -6.54 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_1FWR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWR-query.scw PDB file : Tito_Scwrl_1FWR.pdb: