Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRALIQRVLEAKVEVDGQTTGE--------IKKGLLVFLGLGKEDTLEKGQKLIDKILKYRIFDDEQGKMGWNVSQANGGVLLVSQFTLMAQTQKGLRPDFGPAMPPSDAKALYEQLVEYTRSQFEN--VQTGIFAADMKVHLINDGPVTFNLT 4NBJ Chain:H ((1-152)) MRVVIQRVKGAILSVR------KELEIISEIKNGLICFLGIHKNDTWEDALYIIRKCLNLRLWNNDNKTWDKNVKDLNYELLIVSQFTLFGNTKKGNKPDFHLAKEPNEALIFYNKIIDEFKKQYNDDKIKIGKFGNYMNIDVTNDGPVT----

General information: TITO was launched using: RESULT: Template: 4NBJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 574 -13528 -23.57 -100.96 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain H : 0.80 3D Compatibility (PKB) : -23.57 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_4NBJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NBJ-query.scw PDB file : Tito_Scwrl_4NBJ.pdb: