Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMSQIVDIRAREILDSRGNPTIEADVILESGVVGRACAPSGASTGSREALELRDGDKSRYLGKGVRTAVQNVNSSIHELLVGQSVFEQKALDEKMIAFDGTENKSKLGANATLAVSLAAAHAAAAEQKLPLFQYIANLRGQTTLTMPVPMMNILNGGAHADNTVDIQEFMIEPVGFTSFAEALRAGAEVFHSLKSVLKKQGLNTAVGDEGGFAPNLRSNEEAITVILQAIEQTGYKAGSDIMLALDCASSEFYKNGQYILEGEGNKSFTSNQFADYLAGLVKQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDLFVTNPKILQRGIDEKVGNSILIKYNQIGTLTETLDAIYLAKANGYTTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEELTKAVYRGKAEFKGLK 5J04 Chain:A ((1-406)) -YGTQIAEITAREILDSRGRPTVEAEVHLEDGSVGLAQVPSGASTGTFEAHELRDDDPSRYGGKGVQKAVENV-SAIEDALIGLSALDQEGLDKAMIALDGTPNKKNLGANAILAVSLATAHAAATSLNLPLYRY---LGGPLANVLPVPMMNVINGGAHADNNVDFQEFMIMPVGAPSFKEALRWGAEVFHALAKVLKDKGLATGVGDEGGFAPNLGSNKEALELLLTAIEAAGYKPGEQVALAMDVASSEFYKNGLYTCDG---VSHEPAGMIGILADLVSQYPIVSIEDGLQEDDWSNWKTLTQQLGSTVQLVGDDLFVTNPDRLQSGIEQGVGNAVLIKLNQIGTLTETLRTIDLATRSGYRSVISHRSGETEDTTIADLAVATRAGQIKTGSLSRSERIAKYNRLLRIE-----------------

General information: TITO was launched using: RESULT: Template: 5J04.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2604 -165168 -63.43 -406.82 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -63.43 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_5J04.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J04-query.scw PDB file : Tito_Scwrl_5J04.pdb: