Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTALDQLKTLTTVVADSSDLEAIRKFRPLDATTNPSLITAAAEQPESKELIEDAYYQAKEEG--YKNDELI-ERTIDILTVKFGVEILKLIEGRVSTEVDTALSYDTEATIQKAHELCELYKGYGIDQSRILIKIASTWEGIQAAKVLEAEGIACNLTLLFGLHQAQACADAKVTLISPFVGRILDWYKKAEGVDSYP-IEKDPGVVSVKKIYTYYKQQNIPTQVMGASFRSIDQVLGLAGCDLLTISPSLLTQLEQDTRTVDAALDANKAKQAEAIVR-PAQDEQSFKDELNHDLMAFQLLQGGVDGFIKARDQLSLLLRQSFGIDAEIKS 3TK7 Chain:B ((25-345)) MQKSVLEQLKQVTMVVADTGDFELIKKYKPVDATTNPSLILKAVKEQKYSNLVAETISKVKANNPDLNSDDLVKEIAIEIL-VSFGIKILDVIEGKVSSEVDARVSFNSATTIDYAKRIIARYESNGIPKDRVLIKIAATWEGIKAAKLLQKEGINCNLTLIFDKAQAKACAEAGVYLVSPFVGRITDWQMQQNNLKTFPAIADDDGVNSVKAIYKLYKSHGFKTIVMGASFRNVEQVIALAGCDALTISPVLLEEL----KNRDEHLEVKLTKN----TQSPQISEADFRWLMNENAMATHKLAEGIRLFTKDTIELENIIKQNL--------

General information: TITO was launched using: RESULT: Template: 3TK7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1740 -6662 -3.83 -21.35 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -3.83 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_3TK7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TK7-query.scw PDB file : Tito_Scwrl_3TK7.pdb: