Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIILLGPPGAGKGTQAQLICKRYNIPQISTGDMLRAAIREGTELGLKAKSVMESGGLVSDELIIGLVKERIAQPDCVNGCIFDGFPRTIPQAEALEKEGISIDHVIEIDVPDEEIVKRLSGRRQHPASGRVYHVVYNPPKVEGKDDETGEDLVQRPDDQEETIRKRLASYHTETEQLVGFYQGRAASGENAPTYDKLDGLRTIEDVQKDLFNILDK 4PZL Chain:C ((26-241)) -RIILLGAPGAGKGTQAKIIEQKYNIAHISTGDMIRETIKSGSALGQELKKVLDAGELVSDEFIIKIVKDRISKNDCNNGFLLDGVPRTIPQAQELDKLGVNIDYIVEVDVADNLLIERITGRRIHPASGRTYHTKFNPPKVADKDDVTGEPLITRTDDNEDTVKQRLSVYHAQTAKLIDFYRNFSSTNTKIPKYIKINGDQAVEKVSQDIFDQLNK

General information: TITO was launched using: RESULT: Template: 4PZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 916 -72822 -79.50 -337.14 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -79.50 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_4PZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PZL-query.scw PDB file : Tito_Scwrl_4PZL.pdb: