Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKPI---SLYIGLRYTRARRSNHFISF-IALVSMVGLTLGVAVLITVLSVMNGFDRELKNRVLGMVPQATVS---------------------------STQILTDW-------------PELVKRVENHPHMTGVAPFTQLQGMLTAQGQVAGIMVTGIDPKYEKNVSIIQNHIVAGSLDSLKKGEFGIVLGKDMADSLGLRLNDSVTLVLPEATPSPAGVVPRFKRFKVVGIFSVGAEVDSMVGYIALYDASTLLRLPDGAQGVRLKLDDIFAAPQVADDIVKNLPSNFYATNWTYTHGNLFNAIQMEKTLVGLLLV----LIIVVAAFNIVSSLVMVVTDKKSDIAILRTLGASPSMITKIF-------MVQGTVIGVIGTVAGTVLGVILALTISDIISWFNNVLGLNLFDAYFVHYLPSYLRWKDVTIIVIVSLLLSFLATIYPALRAAKVQPAEALRYE 4OJD Chain:H ((14-490)) RAEPPHCSKTPIVRAQTSQNAMGMQMQFSIGLHTAVCFRLYSLLHTIRLEKLEHHHPITQRYTFG-IPEVHASCICECDATSSTCTAESHQFTACPEDETSSCYRTFFPNQTPIGCSEDDIPKLCCDVRFKPYKNMTFLAVKLEQPTTYATFVYAAYDFVNGYWVEKDKTKIRSQLDGGTQDRHLDQKRRISLAVTAGARASHQLETGMYFSRTSNGGET-------EELRMQPLNEITDNNFDRLGWYRM-DDSGHFHVN----NGVVKMEEIHKA-KVKNCKEQTYKSILSANHYMPGHFNLTRPLEVIKPWIQSARIFDSSLRQAVVTHAEGTNLQISIHLESQNLVFFHNASRIRDFSGSIIVDSKSNRLFNLTVYEASGKIDGSVKMS-TGFGSDTIHTFTAYVSDLHASNRSMIIPLPAIVGQGARAICLRADSM-ADIDKICHVIEYFESPLF------

General information: TITO was launched using: RESULT: Template: 4OJD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1923 -25176 -13.09 -64.72 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain H : 0.65 3D Compatibility (PKB) : -13.09 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.137

(partial model without unconserved sides chains): PDB file : Tito_4OJD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OJD-query.scw PDB file : Tito_Scwrl_4OJD.pdb: