Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDATLIANDAKNIVQAHLDRLGEPKDNRLSEEAKQQKFAQIEAELKKRDAVLVAHYYCDPDVQELAEKTGGCVSDSLEMARFGRDHAASTLVVAGVKFMGETAKILSPEKTILMPTLEATCSLDLGCSVDEFTAFCDQHPDHTVVVYANTSAAVKARADWVVTSSCAVEIVEHLDSLGEKIIWAPDQHLGRYIQKKTGADM--LLWDGACIVHEEFRARGIANMKALYPDAAVLVHPESPESVVEIADAVGSTSQLIKAAQTLPHQRLIVATDRGIFYKMQQAVPVKILVEAPTAGEGATCRSCAHCPWMAMNELDGILEVLQKGDQEIHVDSALAERAKMPLDRMLAFSASLKR 5F33 Chain:A ((8-305)) -------------------------------------MVDEILKLKKEKGYIILAHNYQIPELQDIA----DFVGDSLQLARKAMELSEKKILFLGVDFMAELVKILNPDKKVIVPDRSATCPMANRLTPEIIREYREKFPDAPVVLFVNSTSECKTLADVICTSANAVEVVKKLD--SSVVIFGPDRNLGEYVAEKTGKKVITIPENGHCPVHQ-FNAESIDAVRKKYPDAKVIVHPECPKPVRDKADYVGSTGQMEKIPERDPSRIFVIGTEIGMIHKLKKKFPDREFVPLE----------MAVCVNMKKNTLENTLHALQTESFEVILPKEVIEKAKKPILRMFELMG----

General information: TITO was launched using: RESULT: Template: 5F33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1757 -97291 -55.37 -328.68 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -55.37 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.622

(partial model without unconserved sides chains): PDB file : Tito_5F33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F33-query.scw PDB file : Tito_Scwrl_5F33.pdb: