Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEEKMNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALKEADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQNQG 3K1S Chain:D ((3-107)) -----AMMTTAEQIPFQLILNSGNARSFAMEALQFAKQGKMAEADEAMVKAKEAINEAHHFQTELIQSEARGEKTEISVLLIHAQDHLMNAITVKELAAEFIDLYKKLEA

General information: TITO was launched using: RESULT: Template: 3K1S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 343 -17816 -51.94 -169.68 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -51.94 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_3K1S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K1S-query.scw PDB file : Tito_Scwrl_3K1S.pdb: