Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNF--SVTPIT-SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDIS---VNSATTNSNVMVEYGKNEIEKDMSLIFDL------------VDYVSYPQIVTLKPKSV--QTVAFHV---RMPNERFDGVLAGGITFQEIQTEKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQNDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTN--FSFPTALNGQPLTPGEYHLTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNNWIYILVASLF------LLFILLCI-----LLFVLRKKEEKEK--------------------------------------------------------------- 4YB8 Chain:A ((1-464)) MNDLERLFNPSAIAVVGASKDPSKIGSQILRNLLSYGFKGKVYPINPTADELMGLKCYPKVSDVPDKVDVAVISVPSDKVLGVIDDCGKAGVKFAVVITSGFKEVGNEELEEELVRRAHSYGMRVLGPNIFGYLYAPARLNATFGPKDVLSGNVAFISQSGALGIALMGYTVVENIGISSIVSVGNKADLDDVDLLDFFDKDPNTGVIMIYLEGIAPGRGRMFIDVASRVSLRKPIIVIKAGRTEVGARAAASHTGSIAGSVAIYESAFKQSGILMAKSVEDAFDWTKALSWNPIPEGERLIVLTNGGGAGVQSTDTFADNGIYLSKPPESLIQEIKKFVPPFASFANPIDITGMAPDDWYYMGTLAALKNPDVDALTVLYCQTAVTTPIGVAKGIVDAIKEAGNSKPVTVGMVGGPEVAEAVSFLNKQRIAAYPTPERASSAMSALYAYARARSYVMKSLAVR

General information: TITO was launched using: RESULT: Template: 4YB8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1983 -10729 -5.41 -29.39 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -5.41 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.106

(partial model without unconserved sides chains): PDB file : Tito_4YB8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YB8-query.scw PDB file : Tito_Scwrl_4YB8.pdb: