Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEFKTEKEKMIAGELYFAN-DPELVADRMFARSQSQIINQAE--SAELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNCMFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGAGSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDTVTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNKMKEE 4EGG Chain:C ((2-187)) -----TEKEKMLAEKWYDANFDQTLINERLRAKVICFALNHTNPVATMMRKVLIDALFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKIDND--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4EGG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 958 -2302 -2.40 -12.58 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -2.40 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_4EGG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EGG-query.scw PDB file : Tito_Scwrl_4EGG.pdb: