Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------MTTEHFDLANPVTKVD--DIPDYEMYSQTIDSLNKRFGN-RVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNG----QFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYL-YDNLALYEYVIDLCLSRGGTLF------------------------------------------- 2YXO Chain:A ((1-263)) MVDSHVHTPLCGHAEGHPEAYLEEARAKGLKGVVFTDHSPMPPWYDPESRMRLEALPFYLLALERVRERAQDLYVGIGLEADFHPGTEGFLAQLLRRYPFDYVIGSVHYLGAWPLDHPDHQEEYAWRDLKEVFRAYFQEVEKAARSGLFHAIGHLD-LPKKFG-HRLPEEALLELAEPALRAVAEAGLFLDVNTAGLRRPAKEVYPAPALLRRARELGIGLVLGSDAHRPEEVGFAFPEVQALLAGLGFREAYYFVEGSPVAYPL

General information: TITO was launched using: RESULT: Template: 2YXO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 801 28071 35.04 156.82 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 35.04 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_2YXO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YXO-query.scw PDB file : Tito_Scwrl_2YXO.pdb: