Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISMKVVFHIDELE----KWSETGKNVKNLIKASPETTIVVSVNGIAITGYLDSAN----AEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYIKP 2HYB Chain:F ((1402-1498)) ----SILHTVNKSPFERNSLESCLKFATE--------GASVLLFEDGIYAALAGTRVESQVTEALG-KLKLYVLGPDLKARGFSDERVIPGISVVD--YAGFVDLTTECDTV---

General information: TITO was launched using: RESULT: Template: 2HYB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 409 -6185 -15.12 -69.49 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain F : 0.65 3D Compatibility (PKB) : -15.12 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_2HYB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HYB-query.scw PDB file : Tito_Scwrl_2HYB.pdb: