Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRRKPMKFMTLNTHSWLEPE----PEKKLQELADKILLEDYEVIALQEINQLLESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEEGNALLT--KKTLES-QVLTVSQF------QKKEDYQTRKILIGKTKID--DQDIFVSSCHFSWWTDKKSGFYFEWKNLENYFLE--TRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEK-GKAATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVNE 3G6S Chain:A ((2-261)) -----TDVRWATFNIRYDNPQDSLNNWQYRKDRVCQFIKDHELDIVGMQEVLHNQFQDLRA---------------------------GL---P-EYDGIGVGRDDGKTAGEYAPLFYRKDKYEVLDSNTFWLAENPDSVGMMGWDAVCVRIATWAKFKDKATGKIFMAVNTHFDHVGEEA--RRQSALLIIRKIKEIVGERPAVVTGDFNVTDASDAYETITTNEFVMKDAYKTAARVTGVDYTFHDFA-RI-PAEDCEKIDFIFVTPQVLVKSCEIPAEV-PEALLSDHNPQLADLELE-

General information: TITO was launched using: RESULT: Template: 3G6S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1309 8827 6.74 36.47 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 6.74 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.301

(partial model without unconserved sides chains): PDB file : Tito_3G6S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G6S-query.scw PDB file : Tito_Scwrl_3G6S.pdb: