Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKENFKRSVSRMNAFRSFQVIKE------------PVFHTAISDLPFTELAENEVLVKIAYSDVNYKDALAMSE----------------SGQV-IRTYPMTPGIDLSGTVVQSNN--PRFSKEDLVLATGFG--------------------LGV-THPGGYSQYQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGVGSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPE-T------------------TKALGKQQIDYVIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPIDQRLSLWQQMADLQIA--DEL-VVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK 4A0S Chain:C ((20-415)) ----------PVPDTYLALHLRAEDADMFKGVADKDVRKSLRLGEVPMPELAPDEVLVAVMASSINYNTVWSAMFEPIPTFHFLKQNARQGGWATRHDQPYHVLGSDCSGVVVRTGIGVRRWKPGDHVIVHPAHVDEQEPATHGDGMLGTEQRAWGFETNFGGLAEYGVVRASQLLPKPAHLTWEEAAVSPLCAGTAYRMLVSDRGAQMKQGDIVLIWGASGGLGSYAIQFVKNGGGIPVAVVSSAQKE-AAVRALGCDLVINRAELGITDDIADDPRRVVETGRKLAKLVVEKAGREPDIVFEHTGRVTFGLSVIVARRGGTVVTCGSSSGYLHTFDNRYLWMKLKKIVGSHGA--N----HEEQQATNRLFESGAVVPAMSAVYPLAEAAEACRVVQTSRQVGKVAVLCMA--

General information: TITO was launched using: RESULT: Template: 4A0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1935 -28066 -14.50 -87.16 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -14.50 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_4A0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A0S-query.scw PDB file : Tito_Scwrl_4A0S.pdb: