Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MDNHTRKLLGLT--------DKH------LFFDEE----WLIEKEYKGVQAQFIKGKLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKV-KYLVMDMNASYCQLLKTV-FPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLL-LKDSTTLEPLKRH----YHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKQFHLRYLSRLKRNSRSSIATNRVFQMPLRCPTQTASLRG 4CZX Chain:A ((1-324)) PHMMDSRDWTQLGCVAYPSPIHPDYHAGPASTIAFDNQDELLWIGTQ--KGFAGSFIGREL-------KRFTAFRI-----------------------------------------------HPETDGPLRQFLFVD----------KGVIFLGSRSVYMAARSGVPIWSIRHES------MQDLRAMSFTSKGTSEILVAGWQNKMLVIDVNKG--EVVKELPTQDQYSFLKMSRYICAATNKGTVNILDPITFTIKKQWQAHGAFINDLDTS-NDFIVTCGGSHRQTHNTPAI----LDPYVKVFDLKNMSAMNPVPFAPLAAHVRMHPRMLTTAIVVNQ--------AGQIHVTDLLNPSNSQVCYTQPQGVVLHFDVS------RTGEGKALADNKHNTYVWGSPNKIQFTE

General information: TITO was launched using: RESULT: Template: 4CZX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1706 30805 18.06 104.07 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 18.06 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.067

(partial model without unconserved sides chains): PDB file : Tito_4CZX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZX-query.scw PDB file : Tito_Scwrl_4CZX.pdb: