Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------M-SHTQLIKDTL-NILDLNIYFEE----------NCLTKEKYKGQICLIYK--------GTLLYKPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSE------YRTYLELKKQRFKCKSCQRTFVADTSVAEKH---CFISQ-KVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFK-SVRSVASAMSFIMMDG-QSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPY----------VSLVKKLFPECSV------DHRS----FPHRPTYWQNFLESSSERNHGTT----------- 5D8M Chain:A ((8-339)) RPVAPLAHAMSPSVLVPAGLAGIQDGRGRFREIMTAIMADHGAFLPGDRSSDGDGILWRLAGEPGPTGRPVPLGVSTAGIRLVLVPGLLAECVSESSLLFDDARPDVERYGYATTLVRTGGRWGSARNAAIIHEVVAKLPENDTIVFVTHSKGAVDVLEALVSYPDLAAR-------TAAVVSVAGAI--DGSPLAETFSDGLLRFAESMPLSSCPPGEGTEALDSLKRAYRLRFLAEHRLPARVRYYSLAAFASREETSAILRPFYDILAKTDALNDGLVIAADAIIPGGTLLGYPNADHLAVAMPFSKKPSLLTSVISKNSYPRPALLEAIARYVEEDL

General information: TITO was launched using: RESULT: Template: 5D8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1080 -29104 -26.95 -111.51 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -26.95 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.159

(partial model without unconserved sides chains): PDB file : Tito_5D8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D8M-query.scw PDB file : Tito_Scwrl_5D8M.pdb: