Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRKSLQTILLKEAISMKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKVTASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSARLDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDTLLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQLGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANGNVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADLVFYLPKRVYDHAAIKLL-NGNIMIET-LEAKDIYTKS-TNGNIIVDQLTATMLEVEG-VNGNIDIRNGNILDSIIETV-NGTVTFGAT-AENLSVS-LVNGDVRLTIKEDNLKKVEASSVNGNVKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQLSTTTGSIYLKDTDK 4OPW Chain:A ((108-225)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DELKINANSNCLVQLKQKVEVGKLDLNVSGSANMVVNELKTDKLECSINGSGTINLKAGNAEEADYSITTDGEIMAFGVAVPEVNCKITGKGSAQIHPTD--NLKATI-VGKGNIRYKGPT-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 680 43942 64.62 395.87 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 64.62 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.408

(partial model without unconserved sides chains): PDB file : Tito_4OPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPW-query.scw PDB file : Tito_Scwrl_4OPW.pdb: