Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQFQGSAKMKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYELDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF 5LFI Chain:A ((27-99)) -------------FENELRSMLATALEK--DISQEERNALNIAEKALDNSE---YLPKIILNLRKALTPLAIN--RTLNHDLSELYKFITSSK-------------------

General information: TITO was launched using: RESULT: Template: 5LFI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 200 -31291 -156.46 -428.64 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -156.46 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.581

(partial model without unconserved sides chains): PDB file : Tito_5LFI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LFI-query.scw PDB file : Tito_Scwrl_5LFI.pdb: