Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTYDAIIIGSGVSGLSAAYGLKEA-GKTVLVVEE-D--------LWGGTCPNRGCDPKKVLLSAVEARNRVKQLSGKGFNEI---PTANWEELQKFKRTFTDPVPESRKKQLA-E-AEIDHLSGTARFLDDSSIEVN---------EEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSLPVLPKEIIFIGGGYIAFELATIANAA---GSKVTIVHHNQRPLKEFEASLVEEAVHQMEASGIQFAFGVETQKIIS-EGTRYRLV-GKETELVADMIFCATGRQPNTESLALEQANIVF-DKHGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPIIPTIVYASPKLAEVGVTKSHASSS-D--QVVEMDL---TSWFTYHRVNEPVAKAELTFDQ-QNYLIGAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK 1FEC Chain:A ((1-470)) SRAYDLVVIGAGSGGLEAGWNAASLHKKRVAVIDLQKHHGPPHYAALGGTCVNVGCVPKKLMVTGANYMDTIRESAGFGWELDRESVRPNWKALIAAKNKAVSGIND-SYEGMFADTEGLTFHQGFGALQDNHTVLVRESADPNSAVLETLDTEYILLATGSWPQHLGIEGDDLCITSNEAFYLDEAPKRALCVGGGYISIEFAGIFNAYKARGGQVDLAYRGDMILRGFDSELRKQLTEQLRANGINVRTHENPAKVTKNADGTRHVVFESGAEADYDVVMLAIGRVPRSQTLQLEKAGVEVAKNGAIKVDAYSKTNVDNIYAIGDVT--DRVMLTPVAINEGAAFVDTVFANKPRATDHTKVACAVFSIPPMGVCGYVEEDAAKKYDQVAVYESSFTPLMHNISGST--YKKFMVRIVTNHADGEVLGVHMLGDSSPEIIQSVAICLKMGAKISDFYNTIGVHPTSAEELCSM--

General information: TITO was launched using: RESULT: Template: 1FEC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2532 10560 4.17 24.39 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 4.17 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_1FEC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FEC-query.scw PDB file : Tito_Scwrl_1FEC.pdb: