Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACVAGNSYGIGASVT-LPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFGPLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGR-TLAAEEDFVQAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFW-LNAPFYPTDRINGTGDSLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE 4C5L Chain:D ((5-267)) ------KVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTMDKDTWSHDVTPLPMDVFEKQLE-TALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYFVVDPVMVCKG-----NPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKKAATIIFDKGAQHVIIKGGKALDQDKS----YDLYYDGQTFYQLTTDMFQ--YNHGAGCTFAAATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIE

General information: TITO was launched using: RESULT: Template: 4C5L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1610 11181 6.94 44.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : 6.94 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4C5L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C5L-query.scw PDB file : Tito_Scwrl_4C5L.pdb: