Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFENKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPEYANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLPKQIPVSGMVERLP-VDHPDVFSVYDKFA--QQNHGALFRSDFHWEEYWRFENEDERTAAVYYDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNEPLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVG-TADAFHFIIEDPVAPWNNGVFALTWDEQGQVRVLN-EPIGKSVRLDIQTLTCLMMNYRRASYLARIERLE-TDEETLKSLERIIPNMEAYFSDYF 4MY0 Chain:C ((4-392)) -----VTDDLRLVDITETQLDDVLRVRARSFGLLAAGAR------EDWVRDA-VEFVHDGRFLGVVSGDEVVAAARIWDFQQWWGGRRVPMAGIAGVVVAPEYRGRGVGSLLMRGVLERSRDKGMPISALYPATTVIYRHLGYEFGGHRYRFSFQAADLRSLGGREVAVRRAGAKDAARFLELVGTAHEASRASGLLVWPESKIAEWLEDEE--N--FA-YL----AEDGFVVYNWSDGDLQVDELVAHSEATARALWATVGSGASIARTVHAYLSPNDPVHLLVEHEADKQAHVQRWMLRLLDAPAAIAARGFAPGAAAEVDLLIDDPGVPAQSGRWHLSVADG-TGELTPSDRSGDVLQLGSRGLAALYAGTP-LAALRTAGLVTGGPVASDRLLDTAFGGAAPYMLDYF

General information: TITO was launched using: RESULT: Template: 4MY0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2066 -116696 -56.48 -304.69 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -56.48 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4MY0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MY0-query.scw PDB file : Tito_Scwrl_4MY0.pdb: