Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEFTNSDSFCHPDG-K---DSVRGR--------LTFT-EDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDINMGCPVPNVAERGKGSGLILRPEVAAQLIEAAKA-GGLPVSVKTRIGYTEMSEMEEWISHLLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGD----IPDRQTGEELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSITGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ 4WFS Chain:A ((6-309)) -----------HHLILAPMVRVGTLPMRLLALDY-GADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFRTCEREQNRVVFQMGTSDAERALAVARLVE-NDVAGIDVNMG-------------ALLSDPDKIEKILSTLVKGTRRPVTCKIRILPSL-EDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAIADTL-----SIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKEGL----RPLEEVMQKYIRYAVQYDN----HYTNTKYCLCQMLREQLE-SPQGRLLHAAQSSREICEAFG-------------

General information: TITO was launched using: RESULT: Template: 4WFS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1504 -41824 -27.81 -153.20 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -27.81 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_4WFS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WFS-query.scw PDB file : Tito_Scwrl_4WFS.pdb: