Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEH--GLKYDFVQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFILSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLA--IDWPIDPSRAIMSEKDHRHPTLKEFEAENPFVYGEI 1OFN Chain:B ((18-183)) -----------------VFADDRGLLILPYQEEAFVEAHGGPLFRVAQTIHSMS-KRGVVRGIHYTVTPPGTAKYVYCARGKAMDIVIDIRVGSPTFGQWDSVLMDQQDPRAVYLPVGVGHAFVALEDDTVMSYMLSRSYVTQDELALSALDPALGLPIDIGVEP---IVSDRDRVAITLAEAQRQG-------

General information: TITO was launched using: RESULT: Template: 1OFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 799 -22618 -28.31 -139.61 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -28.31 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1OFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OFN-query.scw PDB file : Tito_Scwrl_1OFN.pdb: