Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLAAFEAFVKVMETGSISVAADQLFITQPAVTKRIHSLEEYFGVKLFESAGRGVQATHAAHSLLPKVKSWLNELRDIHHTLSHEQTQIQGRLKIGTSHHIGLHHLPAPLKHFVQQFPQVTLDVHFVDSEQAHEQVLAGDLELAFLTLPPSGDERLNYITIWN-DPLVFVAAPFHPLAQKKNLILQDLIEYPSLLPAAQTYTAQITLAEFEKQCLKPKITMSNNPLESIRMLVSIGLGWSVLPKTLLN------QDMQQLDLN-VEMNRQLGMVWHPARTQSRAMQELIKMMQE 3FZV Chain:D ((4-295)) YTLRQLKYFVTTVEC---AEASRKLYIAQPSISTAV--LEESF-------------LTPAGARFYRKAQELLRMAHEFEQ-----NDVIAGQIDIGCFETVAPLYLPGLIAGFRQAYPGVEIRIRDGEQQELVQGLTSGRFDLAFLYEH-DLDSTIETEPLMPPQRPHALLPEGHRFAGQAQVSLRDLCLEPMILLDVQ-PSRTYFVSLFEELGLTPNIAFSSPSIEMVRGMVGQGFGFSLLVTRPHSECTYDGKKVVMVDLAEPVSTSGLAAAWLKRAQLTKPARLFVDYCR-

General information: TITO was launched using: RESULT: Template: 3FZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1107 -91679 -82.82 -352.61 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -82.82 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.577

(partial model without unconserved sides chains): PDB file : Tito_3FZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZV-query.scw PDB file : Tito_Scwrl_3FZV.pdb: