Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNHENYWTFCPHNLFWIDRKINRIDMSQTTQTDIITLGDVATRLPSFIPKVPHILNGLKQAYLRTSNTPTGLGIAFEKAVKRNPKGTALLFED-----QSYSYEALNEWANQISHYYLSLGARKGDVIAVMIENRSELIATIVGLAKIGVTIALVNTSQVGKVLAHSINLVKPIAVIAGEEVRAAIDE---------IRQDLNVPKDRFHWFADQATRQKSGTAPKGYVNLADQIDQFPKFNPSTTRSVTGNDGLFYIYTSGTTGLPKAVIFKHSRWT-LAYGTYGHILNLGPDDVMYVTLPLYHATGVVVCWCGVIAGSATLAIRRK----YSTSAFWKDVQKFNASAIGYVGELCRYLMDAPVTE--LDRNHRVTKM--IGNGMRPNIWDKFKQRFG----VKEVLELYASSEGNVGFSNIFN-F---DNTVGFSPTPYAIIQFDKEKNEPIRDKNGWCQKVKAGEVGLLIGKITSR--SPF-DGYTDPEKNKSVIWKDVFKKGDSYFNTGDLVRDIGFRHAQFVDRLGDTFRWKGENVSTTEVENMVCEYDKIAEAVVYGVEIPNTNGRAGMAAITLAD-GEELNDADLTEMVTVFKKCLPTYAVPVFLRVQKKVETTGTFKYQKNKLKEEAFNPSKTSERLLALLPGASSYCDITTEIFDNIQAYKYRF 4WD1 Chain:A ((91-654)) ----------------------------------------------------------------------LNYAEHALRAAGTRPDEPALLYVDETHEPAPVTWAELRRQVASLAAELRALGVRPGDRVSGYLPNIPQAVVALLATAAVGGVWTSCAPDFGARSVLDRFQQVEPVVLFTVDGYRYGGKEHDRRDTVAELRRELPTL-RAVIHIPLLGT-----EAPDGTLDWETLTA-AD--AEPVYEQVPFDHPLWVLYSSGTTGLPKAIVQSQGGILVEHLKQLGLHCDLGPGDRFFWYTSTGWMMWNF--LVSGLLTGTTIVLYDGSPGFPATDAQWRIAERTGATLFGTSAAYVMACRKAGVHPARDLDLSAIQCVATTGSPLPPDGFRWLHDEFAAGGADLWIASVSGGTDVCSCFAGAVPTLPVHIGELQAPGLGTDLQ---------SWDPSG--DPL-TDEVGELVV--TNPMPSMPIRFWNDPDGS--RYHDSYFDTYPGVWRHGDWITLTSRGSVVIHGRSDSTLNR--VRMGSADIYEAVERLPEIRESLVIGIE------YW-MPLFVHLAPGATLDDALLDRIKRTIRVNLSPRHVPDEVIEVPGIPHTLTGKRIEVPVKRLLQGTPLD-K----AVNPGSIDNLDLLHFYEELA------

General information: TITO was launched using: RESULT: Template: 4WD1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3065 -16441 -5.36 -31.50 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -5.36 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_4WD1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WD1-query.scw PDB file : Tito_Scwrl_4WD1.pdb: