Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGPDMQAIILDTETHTLNGL---PIEIAYAPIEINAGKLTLDKSKLFDQLYQVG--TPISYAAMAVHHILESDL-ENQPHYKTFK----LPDT---------TTYIIGHNIDYDIAAIARC----GVD---VSHIKPICTLALTRKTWPDAEAHNISALIYMISQGSSKARELLKGAHRADADIILTANILMHIVYHLNIHDIEELYRVSEEARIPTTINFGKHKGTAIAELPKDYIQWLLRQDELDVYLRKALESAF 3V9W Chain:D ((35-224)) ----RGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQG-WLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSGYEALHEIFKVVRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG---QTVLSKACQTAGMDFD-----STQAHSALYDTERTAVLFCEIVNRWKRL---------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3V9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 724 -56136 -77.54 -342.29 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.63 3D Compatibility (PKB) : -77.54 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_3V9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V9W-query.scw PDB file : Tito_Scwrl_3V9W.pdb: