Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMKLLMTSVLSTSLFLVACGGGSSDDGPATTNPSGTPTNNIQNPVVKVEAYTSTNLGSVAAESSILTYKMLGQSGQEVQATSLVFTPNTPPPVGGWPIVVWAHGTTGVADACAPSKAALADSTKDLISKLLAAGYVVVAPDYEGLGTPGI--HPFLNVKSEAFSITDAVVAARNYLSQRNLLTSKKWGTVGHSQGGHAALGAAQYANRAQ--LDYKGTVAVAPASNWGSILVDGEAQVANAPIDIKIGTYAQLDTYTALVTAGIRNTQPS-FDYGQVFTSQISSIAAQAENLCSGPLYGAFYEGMS---NYAKDHNGTLDGFTRTQP--NFMA-VPLVKTFLEKDSQPLQVKVTTPIIIYQGIADPTVPKLATDLLISNATVVGTKINSYVTGNWDHGTAMSS-NVDNIVGNVQTLLSAQ 4EZI Chain:A ((38-371)) ----------------------------------------------------------HYDLQLYKINYKTQSPDGNLTIASGLVAMPIHP--VGQVGIISYQHGTRFERND-VPSRN-NEKNYIYLAAYGNSAGYMTVMPDYLGLGDNELTLHPYVQAETLASSSIDMLFAAKELANRLHYPISDKLYLAGYSEGGFSTIVMFEMLAKEYPDLPVSAVAPGSAPYGWEETMHFVMLE----P-------GPRATAYLAYFFYSLQTYKSYWSGFDEIFA-------PPYNT----LIPELMD-GYHAVDEILQALPQDPLLIFQPKFSNGIISKTDRNTEILKINFNHYDFKPTAPLLLVGTKGDRDVPYAGAEMAYHSFRKYSDFVWIKSVSDALDHVQAHPFVLKEQVDFFKQFER--

General information: TITO was launched using: RESULT: Template: 4EZI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1879 -44439 -23.65 -138.01 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -23.65 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_4EZI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EZI-query.scw PDB file : Tito_Scwrl_4EZI.pdb: