Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFSRKQFALSAIFVAILATGGSFMLLHENADAKAAPTAAQQAATVDVAPVVSKTITDWQEYSGRLEAIDQVDIRPQVSGKLIAVHFKDGSLVKKGDLLFTIDPRPFEAELNRAKAQLASAEAQVTYTASNLSRIQRLIQSNAVSRQELDLAENDARSANANLQAARAAVQSARLNLEYTRITAPVSGRISRAEVTVGNVVSAGNGAQVLTSLVSVSRLYASFDVDE-QTYLKYISNQRNSSQV--------PVYMGLANETGFTREGTINSIDNNLNTTSGTIRVRATFDNPNGVLLPGLYARIRLGGGQPRPAILISPTAVGVDQDKRFVVVVDAKNQTAYREVKLGAQQDGLQIVNSGLQAGDRIVVNGLQRIRPGDPVTPHLVPMPNSQITANATPQPQPTDKTSTPAKG 1T5E Chain:K ((29-259)) -----------------------------------------------------------TELPGRTNAFRIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQA-------QRYKLLVADQAVSKQQY-------ADANAAYLQSKAAVEQARINLRYTKVLSPISGRIGRSAVTEGALVTNGQ-ANAMATVQQLDPIY--VDVTQPSTALLRLRRELASGQLERAGDNAAKVSLKLEDGSQYPLEGRLEFSEVSVDEGTGSVTIRAVFPNPNNELLPGMFVHAQL-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1T5E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 875 -33607 -38.41 -151.38 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain K : 0.68 3D Compatibility (PKB) : -38.41 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_1T5E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T5E-query.scw PDB file : Tito_Scwrl_1T5E.pdb: